A. What would the CsCl gradient pattern from the Meselson-Stahl experiment look like if replication were fully conservative, that is, if a parental strand gave rise to one daughter with all original DNA and a second daughter with all new DNA?
B. Almost all active transcription units are oriented so that they are expressed in the same direction as the replication fork. Why do we think the other transcription units are in the opposite orientation and infrequently expressed?
C. Why is it important for the cell to detect whether or not a replication origin has replicated?
D. How does DnaA initiate replication, yet prevent a second round of replication?
E. Why do bacteria have only one ori whereas eukarotes have many per chromosome?
F. Why would organisms like Drosophila need such high rates of embryonic replication?
G. Why do origins of replication contain a core of A-T-rich DNA?
H, What mechanism do you think could allow some proteins to have long half-lives and other proteins to have short half-lives?
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