Evidence indicate that oocyte maturation or germinal vesicles breakdown (GVBD) in amphibians is induced by progesterone in a time and doses dependent manner.
Hypothesis: progesterone is able to also induce GVBD in fish.
Experiment:
Materials: fish ovarian oocytes, progesterone (dissolved in EtOH) various doses (1; 10; 100 µg/ml), culture media, room temperature, culture time = 24Hr.
What are the controlled, dependent, and independent?
How would you design a controlled experiment?
What is/are the control group and experiment or test groups?
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