You are working in a lab studying trans-membrane proteins and have been able to purify a protein that the lab has been interested in for a long time.
You conclusively show the protein is an integral membrane protein with the COOH terminus located inside the cell and the NH2 terminus on the outside. You also clone the gene encoding this protein.
You lab director singles you out for praise saying “Your ambition is exceeded only by your talent”. Great things are now expected of you. Your next experiment is to examine the distribution of the protein by fluorescence microscopy.
Briefly state how you would specifically visualize this protein using this technique.
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