Schoolcraft College Tracking Food Borne Pathogens Project Questions Paper 2 assignments for lab (june 5)part 2 of project questions for the topic we picked which was tracking food borne pathogens (may 31) Name ____________________________
Lab 3-10 Bacterial Motility: Wet Mount and Hanging Drop Preparations
Read the lab protocol found on pages 211-216 of Microbiology Laboratory Theory and Application by Leboffe and Pierce.
It’s important that you read the lab and data sheets to understand the scope of the lab activity.
1. How is a wet mount prepared? Using your own words, describe it to someone who is unfamiliar with the process.
2. How is a hanging drop prepared? Using your own words, describe it to someone who is unfamiliar with the process.
3. Do you use the same type of glass slide with a wet mount and a hanging drop? Explain.
4. What is the benefit of viewing specimens with a hanging drop compared to a wet mount?
5. What is Brownian motion?
6. Why do you need to be aware of Brownian motion when viewing specimens?
Watch this clip Bacterial Mobility and describe the shape and motility of the two species shown. Pay attention to the
Observations listed on pg 211 in Leboffe and take notes on whether the bacteria are exhibiting true motility or if the
movement you see is influenced by other factors (and if so, identify them).
Staphylococcus epidermidis
Enterobacter aerogenes
Name ____________________________
Lab 5-24 Motility Agar
Read the lab protocol found on pages 401-404 of Microbiology Laboratory Theory and Application by Leboffe and Pierce.
It’s important that you read the lab and data sheets to understand the scope of the lab activity.
1. In your own words, provide a thorough description of a) how the test works (describe it in detail), and b) what it can tell
us about the organisms.
2. Use color to sketch and describe the outcomes of the motility stabs for a motile organism (+ motility) and non-motile
organism (-).
+
Motility
–
3. Consider the tube stabbed with the sterile inoculating needle.
a. Is this a positive or negative control?
b. What information is provided by the sterile-stabbed tube?
4. Why is it important to carefully insert and remove the needle along the same stab line?
Motility
After your topic has been approved, write a blog post to educate your classmates on the problem. Provide clear
context for the problem that catches your classmates up-to-speed and clarifies why it’s important.
What is the problem?
Is there any historical context for this problem? Is it a new problem?
Why is this problem important?
Why are we interested in studying this problem?
What can we gain from studying this problem?
What are the potential solutions to this problem, or what research is currently being done to address this problem?
At the end of your blog post, please briefly reflect on your learning goals for this assignment. What do you think you
will gain from this exercise? What do you hope to gain from this exercise?
Provide in-text citations to your sources for this assignment. It should be clear that you are using reputable sources
for your research on this topic. Provide at least 5 reputable sources at the end of your blog post in the “Works Cited”.
Due Sunday, June 7. I will be sharing an example blog post on the topic l identified on Sunday May 31.
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