1. Consider two parents. One parent does not have the insertion in either of version of her chromosome 16. The other parent does have the insert in both versions of hischromosome 16. If you were provided with DNA from their offspring, how many bands would you expect to see if you performedthis PCR? Would all offspring have identical banding patternson the gel? Why or why not?
2. you heated the samples containing your cheek cells. Think carefully about where this DNA was packaged in your cells, and what high temperatures usually do toenzyme structure and function. What functions doyou think these high temperature incubations served in your DNA extractions?
3. you aIDed a “PCR Master Mix” to your DNA to beginthe PCR. What ingredients did this PCR master mix have to contain to allow the successful amplification ofyour DNA? What do each of these ingredients do in your reaction?
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