BIO 250L Straighterline Ecology Control and Industrial Microbiology Lab Report I have uploaded the files below they contain questions on them from microbio

BIO 250L Straighterline Ecology Control and Industrial Microbiology Lab Report I have uploaded the files below they contain questions on them from microbiology “Lab 6 Microbial Ecology”
“BIO250L”
Student Name: Click here to enter text.
Access Code (located on the lid of your lab kit): Click here to enter text.
“Experiment 1- Mold Growth on Bread and Fruit- What are Optimal Conditions for Fungal
Growth?”
“Table 1: Growth Results”
“Extent of Growth”
“Condition”
“Day 2”
“Day 4”
“Day 6”
“Day 8”
“Day 10”
“Day 12”
“Day 14”
“BrightControl”
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“BrightWater”
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“BrightSugar”
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“BrightLemon”
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“BrightVinegar”
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“DarkControl”
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“DarkWater”
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“DarkSugar”
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“Lab 6 Microbial Ecology”
“BIO250L”
“DarkLemon”
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“DarkVinegar”
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“Table Key:”
“0 = no growth”
“1 = little/some growth (1-25%)”
“2 = moderate growth (26-50%)”
“3 = heavy growth (51-75%)”
“4 = extensive growth (76-100%)”
“Post-Lab Questions”
“1. Which condition produced the most mold growth? The fastest? The least? The slowest?”
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“2. Based on your results, what conditions are most favorable for mold growth on bread and apples?”
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“3. Would these conditions apply to all fungal growth? Why or why not?”
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“4. How would changing the type of bread (fresh from a bakery, no preservatives vs. prepackaged with
preservatives) affect the results? Describe an experiment that would test your hypothesis.”
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“5. How would changing the incubation temperature of the bags affect the results? Describe an
experiment that would test your hypothesis.”
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“6. Look up the pH of lemon juice and vinegar. Based on your results and your knowledge of favorable
environmental conditions for fungal growth, what can you conclude about the effect of pH on growth?
How would making the pH more basic affect growth?”
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“7. What is the source of the mold that grew on the samples?”
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“Insert photo of your cultures after incubation with your name clearly visible in the background:”
“Lab 6 Microbial Ecology”
“BIO250L”
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“Experiment 2- Fluid Thioglycollate Medium to Assess the Effect of Oxygen on Bacterial
Growth”
“Table 2: Experiment 2 Results”
“Sample
Location”
“Growth Location in FTM”
“Oxygen Category”
“Control”
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“Skin”
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“Nose”
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“Throat”
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“Shoe”
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“Post-Lab Questions”
“1. What does the pink band in the FTM indicate?”
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“2. What would be the effect of using FTM tubes with broad pink zones?”
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“3. Provide an explanation as to why bacteria from each location displayed its pattern of growth?”
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“4. Why is it important to pull the inoculating needle out of the FTM via the same path you inserted it?”
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“Lab 6 Microbial Ecology”
“BIO250L”
“5. How would you explain the presence of bacterial growth in the control FTM tube?”
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“Insert photo of your cultures after incubation with your name clearly visible in the background:”
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“Lab 7 Microbial Control”
“BIO250L”
Student Name: Click here to enter text.
Access Code (located on the lid of your lab kit): Click here to enter text.
“Experiment 1- Effect of Chemical Germicides on Bacterial Growth”
“Table 3: Experiment Results”
“Germicide”
“Sample”
“Skin”
“Nose”
“Throat”
“Shoe”
“Zone of Inhibition (mm)”
“Relative Effectiveness”
“10% Bleach”
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“70% Isopropyl
Alcohol”
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“Hibiclens™”
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“Control”
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“10% Bleach”
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“70% Isopropyl
Alcohol”
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“Hibiclens™”
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“Control”
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“10% Bleach”
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“70% Isopropyl
Alcohol”
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Click here to enter text.
“Hibiclens™”
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Click here to enter text.
“Control”
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Click here to enter text.
“10% Bleach”
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Click here to enter text.
“70% Isopropyl
Alcohol”
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“Hibiclens™”
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“Control”
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“Post-Lab Questions”
“1. Which germicide was the most effective? Was the same germicide most effective for bacteria
isolated from the different sources? What could explain this finding?”
“Lab 7 Microbial Control”
“BIO250L”
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“2. Which germicide was the least effective? Was the same germicide least effective for bacteria
isolated from different sources? What could explain this finding?”
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“3. Were any bacteria from a specific source most resistant to the different germicides? If so, which
source?”
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“4. Did the dry filter paper serve as a positive or negative control?”
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“5. Look up the chemical formulas for bleach, isopropyl alcohol, and chlorhexidine gluconate (the active
ingredient in Hibiclens). How does each chemical achieve its germicidal properties?”
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“Insert photo of your cultures after incubation with your name clearly visible in the background:”
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“Lab 7 Microbial Control”
“BIO250L”
“Experiment 2- Measuring Antibiotic Resistance”
“Table 4: Experiment Results”
“Antibiotic”
“Zone of Inhibition (mm)”
“Relative Effectiveness”
“Ampicillin”
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“Kanamycin”
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“Penicillin”
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“Control”
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“Post-Lab Questions”
“1. Which antibiotic was most effective in killing the bacterial lawn? Which was the least effective?”
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“2. What is the mechanism of action for each of the antibiotics used in this experiment?”
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“3. If a population of bacteria evolves resistance to penicillin, are these bacteria more likely to be also
resistant to ampicillin or kanamycin? Explain your reasoning.”
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“Insert photo of your cultures after incubation with your name clearly visible in the background:”
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“Lab 7 Microbial Control”
“BIO250L”
“Lab 8 Industrial Microbiology”
“BIO250L”
Student Name: Click here to enter text.
Access Code (located on the lid of your lab kit): Click here to enter text.
“Experiment 1- Yogurt Preparation”
“Table 2: Yogurt pH Results”
“Sample”
“pH”
“Step 2”
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“Step 6”
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“Step 10”
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“Step 14”
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“Step 15”
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“Post-Lab Questions”
“1. Make a graph of the pH changes over the course of the experiment. How does the pH change at
each step? Why?”
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“Insert graph here:”
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“Lab 8 Industrial Microbiology”
“BIO250L”
“2. What attributes does lactic acid confer to yogurt?”
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“3. How does the consistency of the milk change during the production of yogurt? What facilitates this
change?”
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“4. What are the breakdown products of lactose?”
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“Insert photo of your pH strips after use with your name clearly visible in the background:”
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“Lab 8 Industrial Microbiology”
“BIO250L”
“Experiment 2- Cloning a DNA Fragment to a Bacterially-Derived Plasmid Vector”
“Table 1: Fragment Lengths”
“DNA
Type”
“Longest Length
(in base pairs)”
“Foreign”
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“Plasmid”
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“Post-Lab Questions”
“1. What is the expected size of the plasmid plus the cut foreign DNA?”
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“2. What type of ends to the enzymes BamHI and EcoRI produce? How does this type of end facilitate
cloning?”
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“3. What enzyme is necessary to permanently link the digested foreign and plasmid DNA together to
form the recombinant DNA molecule? How does this enzyme work?”
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“4. How would you clone a gene into a plasmid if there were no common restriction sites between the
two DNA sequences?”
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