# What fraction of total population is strain a and strain b | Homework Help

Consider a culture into which two E. coli strains are inoculated. Strain A is normal with g=21 min at 37 degrees C, and 42 mins at 27 degrees C. Strain B has a temperature-sensitive mutation, such that g=55 mins at 37 degrees C, and 39 mins at 27 degrees C.

We inoculate equal amounts of cells (1 ml of each starter culture whose population is 1 x 10^6 cfu/ml) into each of two separate 1 liter volumes of LB broth at time=0. One of the flasks is incubated at 37 degrees C, one at 27 degrees C.

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Assuming constant exponential growth (i.e. no lag phase, and no exhaustion of nutrients), answer each of these questions:

1) After 6 hours of growth, what is the final concentraion of each strain in each flask?

2) What fraction of the total population is Strain A and Strain B in each flask?

3) How long would each flask need to go for one strain to become 90% of the population?

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