Research Methods Effects And Treatment Of The Abiotics With Anova Test I need you to write the method of our experiment I have attached the time line and w

Research Methods Effects And Treatment Of The Abiotics With Anova Test I need you to write the method of our experiment I have attached the time line and what exactly we did and rubric please be clear and follow the instructions How to Write a Methods Section:
General:
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Explain your methods in a way that is clear, but concise.
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Someone reading this should be able to replicate the experiment
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2-4 pages
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Don’t be too specific. Just give what is needed to replicate and fully understand
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OK to say “We…”. This helps use active voice
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Even though you have not completed your experiments, write your methods as if you have
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Experimental Design, Why, What, When, Where, How
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Experiment design:
Include a table that shows your experimental design
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Include both factors
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All levels of both factors
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The number of replicates
Link methods to objectives/hypotheses
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clearly show which methods test which hypothesis
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Use outside sources to justify the levels used for each factor
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Also use outside sources if you used a previously published protocol
Only discuss this if it is important.
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It may matter depending on the life cycle of your organism, or the time it takes for pots to
dry, etc.
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Ask: would it matter if someone performed this activity at a different time/season/year?
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You may talk about the timeline such as:
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“Germination occurred 3 days after planting”
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• “10 days after germination, we noticed/did…”
Where: Only if it is is important/relevant
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In a greenhouse. In a growth chamber
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How were specific treatment applied?
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This is where you want to be most specific!
How did you measure each dependent variable?
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Again, be specific but concise!
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BUT: Don’t list materials or steps
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Write in paragraph form with complete sentences
You haven’t done this yet, but you should be able to write this section.
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What does a 2-way ANOVA test?
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Relate back to your three objectives, and mention each dependent variable
What effects did you include in your ANOVA model?
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Main effects (each factor on its own)
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Interactive effect of both factors
What other test might you use to compare levels?
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TUKEY KRAMER HSD?
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What is your significance threshold?
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What programs/software did you use?
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Cite SAS JMP
Where: LSB green house
When 3/5/2019 to 4/2/2019
Day one: Fourty-eight pots were obtained, twelve of each pot for each experimental group. The
first experimental group is the low temperature (21.1C) with low nitrogen solution, the second is
the low temperature (21.1C) with high nitrogen solution. The other two experimental groups is
high temperature (26.7C) with low nitrogen solution and the final group is high temperature
(26.7C) with high nitrogen solution. Approximately two to three brassica rapa seeds were placed
into each pot. The containers containing the pots have clear plastic lids on them and they were
placed in the greenhouse in their required temperature situation
Day four: There was condensation seen on top of the plastic coverings. There was at least seven
plants in each treatment group that have sprouted up and have growth. The soil was still very
damp, and the plants were only slighlty misted with deionized water. Excess amounts of
deionized water was added into the basin that holds the pots.
Day seven. There was condensation seen on top of the plastic covering. There was still nice
growth seen with the plants. The plants were watered with deionized water and were left on
their various temperature pads
Day eight. There was condensation seen on top of the plastic covering. There was still nice
growth seen with the plants. The soil was still moist, and the plants only required a slight misting
of deionized water.
Day nine. There was condensation seen on top of the plastic covering. There was still nice
growth, and there was almost one plant per pot, with a few containing 2-3 plants in a pot. The
soil was still moist, and the plants only needed slight watering of deionized water.
Day eleven. Plants were rehoused and waiting to be placed into their various treatment groups
on Tuesday. Currently there are 31 good plants, and another 11 plants that are borderline living.
There is enough for at least seven replicates per group. The plants were individually planted into
small 2″ round pots (Used potting soil)
. The plants were removed from the heating pads to get good growth prior to being placed into
their various groups. Also the plastic covering was removed from the plants as we saw a few of
the plants died possibly from leaving that top on too long after germination took place.
Day fifteen. (DAY ONE TREATMENT) Nitrogen solution was made for the two groups of plants
that are going to be recieving nitrogen-rich water. The Nitrogen solution was made from a pellet
form, and dissolved in deionized water. The nitrogen pellets are 44% nitrogen per 3.8 liters, if
one table spoon of the pellets are added. We made 10L of nitrogen-deionized water, a total of
28.78 grams was added to make the the 10L solution 44% nitrogen. The plants were placed into
their various treatment groups. Each treatment group has its own plastic basin, so over
weekends, 1-2L of either nitrogen-deionized water or deionized water will be added to it for the
plants to uptake during the days that we cannot make it in to water them. Plants 1-7 are in the
21.1C (70F) and given 10mL of deionized water everyday. Plants 8-14 are in the 21.1C (70F) and
given 10mL of nitrogen-deionized water everyday. Plants 15-21 are in the 26.7C (80F) and given
10mL of deionized water everyday. Plants 22-28 are in the 26.7C (80F) and given 10mL of
nitrogen-deionized water everyday. The plants were also measured today as their starting
measurement, and they will be measured everyday that they are given 10mL of their treatment
Day sixteen. (DAY TWO TREATMENT) The plants were measured, and the data points were added
to the excel sheet. The plants were then given their various treatments, being either watered
with 10mL of deionize water or 10mL of nitrogen-deionized water. The plants were left on their
various heating pads, depending what group they were in.
Day twenty-nine. Plants were harvested. Growth rate was calculated with the daily measuring
results. Root length was measured in cm. Dry root biomass was measured in grams. Stomatal
density was measured in quantity of stomatas (number seen under a microscope).
Photosynthetic pigments were measured by measuring the amount of chlorophyll a, b, total
chlorophyll, and cartenoids.
Methods
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Quality Score*
Completeness (repeatability by reader)
Clarity and organization
Appropriateness of experimental design
Description of appropriate analyses
Quality Score*
D1
Use your computer’s spell-check cappaaabilities (!) to catch misssspelled wirds…
(D) Methods: This section is a detailed verbal description of the why, what, when, where, and how
of your experiment. The purpose of the narrative should be to make it clear enough to the reader that
s/he could repeat the experiment based on what is included in this section. DO NOT PROVIDE
LISTS OF MATERIALS! DO describe the statistical techniques used as well as the experimental
procedure. Generally the text is 2 – 4 pages for this section. You may include a figure showing your
experimental apparatus, if it is unique to your study (otherwise describe by model or by reference
to another study). You may also use a figure to show your experimental layout (how you placed
your replicates in certain places and what treatments they received), but again, often these can be
described adequately in words.
Additional Tips for Methods:
•Turn in your Methods section the week of March 25 in lab. You will receive feedback, but try to
do an excellent job, following the instructions for Methods section your TA outlined in the
previous week.
•Now is the time you will benefit from having taken good notes during your experiment – if you
don’t have good notes, consult your collaborators and help each other by sharing dates when certain
things were done or measured. There’s nothing more embarrassing than having members of the
same lab group describe the same experiment using completely different facts about the methods!
Only one version is correct. Remember this is science, so accuracy is of extreme importance
because if someone were to ever repeat your experiment, it would be impossible without the correct
details.
•Some find that writing methods in chronological order can contribute to the ‘storyline’ being
developed by the paper. Sometimes this approach can be clearer to both the writer and reader in
reconstructing what was done.
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