In a restriction mapping experiment using the plasmid | Homework Help
In a restriction mapping experiment using the plasmid P(ST), you cleave the circular plasmid with the restrictionenzymes XbaI, Asp718, BamHI and EcoRI individually and in variouscombinations with each other. You obtain DNA fragments with thefollowing sizes:
Enzymes Used Size of DNA fragments(bp)
BamHI, EcoRI, XbaI 3000, 1850,1250
Asp718, BamHI,XbaI 3000, 2500, 600
Asp718,BamHI 5500, 600
Asp718,XbaI 3600, 2500
BamHI,XbaI 3100, 3000
Asp 718 6100
BamHI 6100
XbaI 6100
Using the above data, make a map of the P(ST)plasmid indicating the distances between each restriction site foreach of the four enzymes.
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