# Determine the cfu/g of soil | Homework Help

20 g soil sample was aIDed to 80 ml of water to make the final volume of 100 ml (tube A). Then, 10 ml of the tube A’ solution was taken and aIDed to 90 ml of water (tube B). 1 ml of the tube B’ solution was taken and aIDed to 9 ml of water (tube C). 1 ml of the tube C’ solution was taken and aIDed to 99 ml of water (tube D). 10 ml of the tube D’ solution was taken and aIDed to 90 ml of water (tube E). Now, 0.1 ml out of the tube C, D or E was taken and plated on agar-media plates in triplicate. The table below shows colony numbers counted, after overnight incubation.

C

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D

E

TNTC (too numerous to count), 431, 378

43, 52, 33

1, 0, 6

(a) What are the fold dilutions in the tubes C, D and E against the tube A?

(b) Determine the CFU/g of soil.

(c) How many bacteria were present in 8 g of soil?

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