BIO 243 HFCC Streak Plate Methods of Isolation Lab Report 1 assingment; lab microbiology – homework due friday 5th junelab book provided as well Name _____

BIO 243 HFCC Streak Plate Methods of Isolation Lab Report 1 assingment; lab microbiology – homework due friday 5th junelab book provided as well Name ____________________________
Lab 1-5 Streak Plate Methods of Isolation
Read the lab protocol found on pages 45-52 of Microbiology Laboratory Theory and Application by Leboffe and Pierce. It’s
important that you read the lab and data sheets to understand the scope of the lab activity.
1. Describe the two culture types and clearly identify the difference between them (mixed culture and pure culture).
2. What is the ultimate goal of using a streak plate methods of isolation? Why do these techniques exist?
3. Does every isolated colony grow from one cell? Why or why not? Explain.
4. Sketch and number the order ​of each movement/streak for each practice plate (indicated as circles below). Use
color for each streak to clearly identify the order in which each movement is made. A movement is delineated by
flaming the loop to sterilize it, see Fig. 1.35.
a. Quadrant Streak
b. T-streak
c. Zigzag (continuous)
d. Identify the circumstances in which each streak method of isolation would be ideal for isolating CFUs. Think about cell
density & culture type.
a. Quadrant
b. T-streak
c.
Zigzag

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