Are you concerned about the undergraduate student finding | Homework Help
1. You have just read a paper that the aIDition of caffeine to cells disrupts the DNA damage checkpoint and you would like to understand why. By searching for proteins that bind to caffeine, you isolate a protein, Caf1. You believe that Caf1 is involved in the DNA damage checkpoint because when you delete Caf1 from yeast cells and aID a drug that causes DNA damage, the cells fail to arrest in the cell cycle.
You give your Caf1-deleted cells to an undergraduate in the laboratory and ask him to take care of the strain for you while you take the weekend off to celebrate your finding. Upon your return, you find the poor undergraduate in tears. He explains that he thinks that he messed up the Caf1 mutant strain while you were gone because after growing Caf1 and wild-type cells in rich media and examining them during mitosis, Caf1 mutants looked the same as wild-type cells. Are you concerned about the undergraduate student’s findings? Why?
2. You are interested in epithelial tissue formation and work in a lab studying Madin-Darby canine kidney (MDCK) cells. MDCK cells spontaneously form hollow epithelial vesicles. Staining these vesicles with an anti-actin antibody demonstrates the existence of apical microvilli near the central cavity of the vesicle. A new postdoctoral fellow in the lab comes to you in a panic. You had given her some dissociated MDCK cells that have been cultured as single cells in a collagen matrix. Because the cells did not look obviously polarized to her, she stained the dissociated MDCK cells to visualize the microvilli and she does not see any signs of polarized microvilli localization in these cells. Explain why you are confident that you did not give her the wrong cell line, and suggest a better experiment that will allow her to test whether the cells you gave her are MDCK cells.
We've got everything to become your favourite writing service
Money back guarantee
Your money is safe. Even if we fail to satisfy your expectations, you can always request a refund and get your money back.
Confidentiality
We don’t share your private information with anyone. What happens on our website stays on our website.
Our service is legit
We provide you with a sample paper on the topic you need, and this kind of academic assistance is perfectly legitimate.
Get a plagiarism-free paper
We check every paper with our plagiarism-detection software, so you get a unique paper written for your particular purposes.
We can help with urgent tasks
Need a paper tomorrow? We can write it even while you’re sleeping. Place an order now and get your paper in 8 hours.
Pay a fair price
Our prices depend on urgency. If you want a cheap essay, place your order in advance. Our prices start from $11 per page.